Embryo cell membrane reconstruction by tensor voting

Image-based studies of developing organs or embryos produce a huge quantity of data. To handle such high-throughput experimental protocols, automated computer-assisted methods are highly desirable. This article aims at designing an efficient cell segmentation method from microscopic images. The proposed approach is twofold: first, cell membranes are enhanced or extracted by the means of structure-based filters, and then perceptual grouping (i.e. tensor voting) allows to correct for segmentation gaps. To decrease the computational cost of this last step, we propose different methodologies to reduce the number of voters. Assessment on real data allows us to deduce the most efficient approach.